Identification of An Unknown Gram Positive and Gram Negative Bacteria

Introduction: The purpose of this experiment is to isolate and differentiate between two bacterial species using Gram’s stain, microscope visualization and different biochemical tests. Bacteria can be classified and differentiated based upon different morphological characteristics, staining reaction, nutritional needs, cultural characteristics, physiology, cellular chemistry, and biochemical tests results for specific metabolic end products.
Identification of unknown bacteria is useful in many situations. It can determine if bacterial contamination is present in food products, blood or tissue samples, water or cosmetics. Then go on to determine what type of contamination is present and give clues as to what type of steps should be taken to solve the problem. Identification of unknown bacteria is also used to screen materials for the presence of new antibiotic, vitamin, solvent and enzyme producing organisms.
This experiment first isolates the gram positive and gr...